Semaphorin 3D (SEMA3D) Mouse Monoclonal Antibody [Clone ID: OTI2H4]

Specifications

Product Data
Clone Name	OTI2H4
Applications	IHC, WB
Recommend Dilution	WB 1:500, IHC 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 200-291 of human SEMA3D(NP_689967) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	89.5 kDa
Gene Name	semaphorin 3D
Database Link	NP_689967 Entrez Gene 108151 MouseEntrez Gene 246262 RatEntrez Gene 223117 Human UniProt ID O95025
Background	Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin. [UniProtKB/Swiss-Prot Function]
Synonyms	coll-2; Sema-Z2
Reference Data
Protein Families	Transmembrane
Protein Pathways	Axon guidance

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