Bile Acid Receptor (NR1H4) Mouse Monoclonal Antibody [Clone ID: OTI4F12]

Specifications

Product Data
Clone Name	OTI4F12
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 21-297 of human NR1H4 (NP_005114) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	nuclear receptor subfamily 1 group H member 4
Database Link	NP_005114 Entrez Gene 20186 MouseEntrez Gene 60351 RatEntrez Gene 9971 Human UniProt ID Q96RI1
Background	This gene encodes a ligand-activated transcription factor, which shares structural features in common with nuclear hormone receptor family, such as a DNA-binding domain that targets the receptor to specific DNA sequences, and a ligand-binding domain, which interacts directly with the ligand and contains a ligand-dependent transcriptional activation domain. This protein functions as a receptor for bile acids, and when bound to bile acids, regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Synonyms	BAR; FXR; HRR-1; HRR1; PFIC5; RIP14
Reference Data
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

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