Nestin (NES) Mouse Monoclonal Antibody [Clone ID: OTI1B4]

Specifications

Product Data
Clone Name	OTI1B4
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:500
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 99-349 of human NES(NP_006608) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	nestin
Database Link	NP_006608 Entrez Gene 10763 Human UniProt ID P48681
Background	This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Synonyms	Nbla00170
Reference Data
Protein Families	ES Cell Differentiation/IPS

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