BLNK Mouse Monoclonal Antibody [Clone ID: OTI2B2]

Specifications

Product Data
Clone Name	OTI2B2
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-292 of human BLNK(NP_037446) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	50.3 kDa
Gene Name	B cell linker
Database Link	NP_037446 Entrez Gene 17060 MouseEntrez Gene 499356 RatEntrez Gene 29760 Human UniProt ID Q8WV28
Background	This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]
Synonyms	AGM4; BASH; bca; BLNK-S; LY57; SLP-65; SLP65
Reference Data
Protein Families	Druggable Genome
Protein Pathways	B cell receptor signaling pathway, Primary immunodeficiency

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