SFRP2 Mouse Monoclonal Antibody [Clone ID: OTI6C2]

Specifications

Product Data
Clone Name	OTI6C2
Applications	IHC, WB
Recommend Dilution	IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 25-295 of human SFRP2(NP_003004) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	31.4 kDa
Gene Name	secreted frizzled related protein 2
Database Link	NP_003004 Entrez Gene 20319 MouseEntrez Gene 310552 RatEntrez Gene 6423 Human UniProt ID Q96HF1
Background	This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]
Synonyms	FRP-2; SARP1; SDF-5
Reference Data
Protein Families	Secreted Protein
Protein Pathways	Wnt signaling pathway

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