PDE1A Mouse Monoclonal Antibody [Clone ID: OTI5H4]

Specifications

Product Data
Clone Name	OTI5H4
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 259-545 of human PDE1A (NP_005010) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	phosphodiesterase 1A
Database Link	NP_005010 Entrez Gene 18573 MouseEntrez Gene 81529 RatEntrez Gene 5136 Human UniProt ID P54750
Background	Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]). [supplied by OMIM, Oct 2009]
Synonyms	CAM-PDE-1A; HCAM-1; HCAM1; HSPDE1A
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction

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