Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: OTI1F11]

Specifications

Product Data
Clone Name	OTI1F11
Applications	IHC, WB
Recommend Dilution	IHC 1:100, WB 1:200 - 1:1000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	marker of proliferation Ki-67
Database Link	NP_002408 Entrez Gene 4288 Human UniProt ID P46013
Background	This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Synonyms	KIA; MIB-; MIB-1; PPP1R105
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS

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