Smad Interacting Protein 1 (ZEB2) Mouse Monoclonal Antibody [Clone ID: OTI15G8]

Specifications

Product Data
Clone Name	OTI15G8
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB2 (NP_055610) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	zinc finger E-box binding homeobox 2
Database Link	NP_055610 Entrez Gene 24136 MouseEntrez Gene 311071 RatEntrez Gene 9839 Human UniProt ID O60315
Background	The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2010]
Synonyms	HSPC082; SIP-1; SIP1; SMADIP1; ZFHX1B
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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