PCNA Mouse Monoclonal Antibody [Clone ID: OTI1H10]

Specifications

Product Data
Clone Name	OTI1H10
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PCNA (NP_002583) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	28.6 kDa
Gene Name	proliferating cell nuclear antigen
Database Link	NP_002583 Entrez Gene 18538 MouseEntrez Gene 25737 RatEntrez Gene 5111 Human UniProt ID P12004
Background	The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Synonyms	ATLD2
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

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