Cullin 4B (CUL4B) Mouse Monoclonal Antibody [Clone ID: OTI7F8]

Specifications

Product Data
Clone Name	OTI7F8
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-300 of human CUL4B (NP_003579) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	103.8 kDa
Gene Name	cullin 4B
Database Link	NP_003579 Entrez Gene 72584 MouseEntrez Gene 302502 RatEntrez Gene 492102 DogEntrez Gene 696410 MonkeyEntrez Gene 8450 Human UniProt ID Q13620
Background	This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Synonyms	CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2
Reference Data
Protein Pathways	Nucleotide excision repair, Ubiquitin mediated proteolysis

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