CNOT4 Mouse Monoclonal Antibody [Clone ID: OTI1F9]

Specifications

Product Data
Clone Name	OTI1F9
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 190-455 of human CNOT4 (NP_037448) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	70.2 kDa
Gene Name	CCR4-NOT transcription complex subunit 4
Database Link	NP_037448 Entrez Gene 53621 MouseEntrez Gene 312227 RatEntrez Gene 4850 Human UniProt ID O95628
Background	The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.
Synonyms	CLONE243; NOT4; NOT4H
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	RNA degradation

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