IRF5 Mouse Monoclonal Antibody [Clone ID: OTI1G7]

Specifications

Product Data
Clone Name	OTI1G7
Applications	IHC, WB
Recommend Dilution	WB 1:4000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human IRF5(NP_116032) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	55.9 kDa
Gene Name	interferon regulatory factor 5
Database Link	NP_116032 Entrez Gene 27056 MouseEntrez Gene 296953 RatEntrez Gene 3663 Human UniProt ID Q13568
Background	This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]
Synonyms	SLEB10
Reference Data
Protein Families	Transcription Factors
Protein Pathways	Toll-like receptor signaling pathway

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