Methylmalonyl Coenzyme A mutase (MUT) Mouse Monoclonal Antibody [Clone ID: OTI2C8]

Specifications

Product Data
Clone Name	OTI2C8
Applications	IF, WB
Recommend Dilution	WB 1:4000, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human MUT(NP_000246) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	79.3 kDa
Gene Name	methylmalonyl-CoA mutase
Database Link	NP_000246 Entrez Gene 17850 MouseEntrez Gene 688517 RatEntrez Gene 4594 Human UniProt ID P22033
Background	This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008]
Synonyms	MCM
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation

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