OTUB1 Mouse Monoclonal Antibody [Clone ID: OTI5B8]

Specifications

Product Data
Clone Name	OTI5B8
Applications	FC, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, FLOW 1:100
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human OTUB1(NP_060140) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	31.1 kDa
Gene Name	OTU deubiquitinase, ubiquitin aldehyde binding 1
Database Link	NP_060140 Entrez Gene 293705 RatEntrez Gene 55611 Human UniProt ID Q96FW1
Background	The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Synonyms	HSPC263; OTB1; OTU1
Reference Data
Protein Families	Protease

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