Proteasome 20S beta 7 (PSMB7) Mouse Monoclonal Antibody [Clone ID: OTI4E3]

Specifications

Product Data
Clone Name	OTI4E3
Applications	IF, WB
Recommend Dilution	WB 1:500~2000, IF 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 58-277 of human PSMB7(NP_002790) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	29.8 kDa
Gene Name	proteasome 20S subunit beta 7
Database Link	NP_002790 Entrez Gene 19177 MouseEntrez Gene 85492 RatEntrez Gene 100686423 DogEntrez Gene 694567 MonkeyEntrez Gene 5695 Human UniProt ID Q99436
Background	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit). [provided by RefSeq]
Synonyms	Z
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Proteasome

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