LIM1 (LHX1) Mouse Monoclonal Antibody [Clone ID: OTI2D5]

Specifications

Product Data
Clone Name	OTI2D5
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 100-362 of human LHX1(NP_005559) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	44.6 kDa
Gene Name	LIM homeobox 1
Database Link	NP_005559 Entrez Gene 16869 MouseEntrez Gene 257634 RatEntrez Gene 3975 Human UniProt ID P48742
Background	This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq]
Synonyms	LIM-1; LIM1
Reference Data
Protein Families	ES Cell Differentiation/IPS, Transcription Factors

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