HLCS Mouse Monoclonal Antibody [Clone ID: OTI1E4]

Specifications

Product Data
Clone Name	OTI1E4
Applications	FC, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, FLOW 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	80.6 kDa
Gene Name	holocarboxylase synthetase
Database Link	NP_000402 Entrez Gene 3141 Human UniProt ID P50747
Background	This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Synonyms	HCS
Reference Data
Protein Pathways	Biotin metabolism, Metabolic pathways

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