FBXO21 Mouse Monoclonal Antibody [Clone ID: OTI2B8]

Specifications

Product Data
Clone Name	OTI2B8
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human FBXO21(NP_269373) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	72.1 kDa
Gene Name	F-box protein 21
Database Link	NP_296373 Entrez Gene 231670 MouseEntrez Gene 360818 RatEntrez Gene 486290 DogEntrez Gene 693647 MonkeyEntrez Gene 23014 Human UniProt ID O94952
Background	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]
Synonyms	FBX21
Reference Data

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