UBE2M Mouse Monoclonal Antibody [Clone ID: OTI2D9]

Specifications

Product Data
Clone Name	OTI2D9
Applications	IF, WB
Recommend Dilution	WB 1:500~2000, IF 1:100
Reactivity	Human, Dog, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human UBE2M(NP_003960) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	20.7 kDa
Gene Name	ubiquitin conjugating enzyme E2 M
Database Link	NP_003960 Entrez Gene 22192 MouseEntrez Gene 361509 RatEntrez Gene 484221 DogEntrez Gene 712206 MonkeyEntrez Gene 9040 Human UniProt ID P61081
Background	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq]
Synonyms	hUbc12; UBC-RS2; UBC12
Reference Data
Protein Pathways	Ubiquitin mediated proteolysis

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