OBFC1 (STN1) Mouse Monoclonal Antibody [Clone ID: OTI2E4]

Specifications

Product Data
Clone Name	OTI2E4
Applications	FC, IF, WB
Recommend Dilution	WB 1:2000, IF 1:100, FLOW 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human OBFC1 (NP_079204) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	41.9 kDa
Gene Name	STN1 subunit of CST complex
Database Link	NP_079204 Entrez Gene 79991 Human UniProt ID Q9H668
Background	OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]). [supplied by OMIM]
Synonyms	AAF-44; AAF44; bA541N10.2; RPA-32; STN1
Reference Data

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