SM22 alpha (TAGLN) Mouse Monoclonal Antibody [Clone ID: OTI8C8]

Specifications

Product Data
Clone Name	OTI8C8
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human reocombinant protein of human TAGLN(NP_003177) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	22.4 kDa
Gene Name	transgelin
Database Link	NP_003177 Entrez Gene 21345 MouseEntrez Gene 25123 RatEntrez Gene 6876 Human UniProt ID Q01995
Background	The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Synonyms	SM22; SMCC; TAGLN1; WS3-10
Reference Data

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