ADH1B Mouse Monoclonal Antibody [Clone ID: OTI5D7]

Specifications

Product Data
Clone Name	OTI5D7
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ADH1B (NP_000659) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	39.7 kDa
Gene Name	alcohol dehydrogenase 1B (class I), beta polypeptide
Database Link	NP_000659 Entrez Gene 707908 MonkeyEntrez Gene 125 Human UniProt ID P00325
Background	The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]
Synonyms	ADH2; HEL-S-117
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism

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