CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI2A10]

Specifications

Product Data
Clone Name	OTI2A10
Applications	FC, IHC, WB
Recommend Dilution	WB 1:200~500, IHC 1:150, FLOW 1:100
Reactivity	Human, Dog, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	34.1 kDa
Gene Name	cytochrome b5 reductase 3
Database Link	NP_000389 Entrez Gene 474479 DogEntrez Gene 714058 MonkeyEntrez Gene 1727 Human UniProt ID P00387
Background	This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq]
Synonyms	B5R; DIA1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Amino sugar and nucleotide sugar metabolism

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