HIBCH Mouse Monoclonal Antibody [Clone ID: OTI2A5]

Specifications

Product Data
Clone Name	OTI2A5
Applications	FC, IF, WB
Recommend Dilution	WB 1:2000, IF 1:100, FLOW 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	39.4 kDa
Gene Name	3-hydroxyisobutyryl-CoA hydrolase
Database Link	NP_055177 Entrez Gene 26275 Human UniProt ID Q6NVY1
Background	This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.
Synonyms	HIBYLCOAH
Reference Data
Protein Pathways	beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation

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