GRASP65 (GORASP1) Mouse Monoclonal Antibody [Clone ID: OTI5G8]

Specifications

Product Data
Clone Name	OTI5G8
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	46.3 kDa
Gene Name	golgi reassembly stacking protein 1
Database Link	NP_114105 Entrez Gene 56082 RatEntrez Gene 485594 DogEntrez Gene 694728 MonkeyEntrez Gene 64689 Human UniProt ID Q9BQQ3
Background	The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq]
Synonyms	GOLPH5; GRASP65; P65
Reference Data

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