RANGAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]

Specifications

Product Data
Clone Name	OTI1B4
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	63.4 kDa
Gene Name	Ran GTPase activating protein 1
Database Link	NP_002874 Entrez Gene 19387 MouseEntrez Gene 362965 RatEntrez Gene 481234 DogEntrez Gene 5905 Human UniProt ID P46060
Background	RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]
Synonyms	Fug1; RANGAP; SD
Reference Data

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