RhoGDI (ARHGDIA) Mouse Monoclonal Antibody [Clone ID: OTI1F2]

Specifications

Product Data
Clone Name	OTI1F2
Applications	FC, WB
Recommend Dilution	WB 1:200~1000, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	23.0 kDa
Gene Name	Rho GDP dissociation inhibitor alpha
Database Link	NP_004300 Entrez Gene 192662 MouseEntrez Gene 360678 RatEntrez Gene 475924 DogEntrez Gene 714752 MonkeyEntrez Gene 396 Human UniProt ID P52565
Background	Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]). [supplied by OMIM]
Synonyms	GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Neurotrophin signaling pathway

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