NIT1 Mouse Monoclonal Antibody [Clone ID: OTI4D12]

Specifications

Product Data
Clone Name	OTI4D12
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35.7 kDa
Gene Name	nitrilase 1
Database Link	NP_005591 Entrez Gene 27045 MouseEntrez Gene 289222 RatEntrez Gene 4817 Human UniProt ID Q86X76
Background	Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
Synonyms	MGC57670
Reference Data

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