HIPPI (IFT57) Mouse Monoclonal Antibody [Clone ID: OTI2G3]

Specifications

Product Data
Clone Name	OTI2G3
Applications	IF, IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:50, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human IFT57 (NP_060480) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	49.1 kDa
Gene Name	intraflagellar transport 57
Database Link	NP_060480 Entrez Gene 73916 MouseEntrez Gene 303968 RatEntrez Gene 55081 Human UniProt ID Q9NWB7
Background	Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1,leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3'present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear
Synonyms	ESRRBL1; HIPPI; MHS4R2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Huntington's disease

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