ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI1B10]

Specifications

Product Data
Clone Name	OTI1B10
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Reactivity	Human, Dog, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	56.6 kDa
Gene Name	ATP synthase F1 subunit beta
Database Link	NP_001677 Entrez Gene 171374 RatEntrez Gene 403669 DogEntrez Gene 713284 MonkeyEntrez Gene 506 Human UniProt ID P06576
Background	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Synonyms	ATPMB; ATPSB; HEL-S-271
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease

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