Protein kinase Y linked (PRKY) Mouse Monoclonal Antibody [Clone ID: OTI3E10]

Specifications

Product Data
Clone Name	OTI3E10
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500, IHC 1:50, IF 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human PRKY expression vector
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	31.7 kDa
Gene Name	protein kinase Y-linked (pseudogene)
Database Link	NP_002751 Entrez Gene 5616 Human UniProt ID O43930
Background	This gene is similar to the protein kinase, X-linked gene in the pseudoautosomal region of the X chromsoome. The gene is classified as a transcribed pseudogene because it has lost a coding exon that results in all transcripts being candidates for nonsense-mediated decay (NMD) and unlikely to express a protein.Abnormal recombination between this gene and a related gene on chromosome X is a frequent cause of XX males and XY females.
Synonyms	OTTHUMP00000033227; protein kinase, Y-linked
Reference Data
Protein Families	Druggable Genome, Protein Kinase

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