BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: OTI3C7]

Specifications

Product Data
Clone Name	OTI3C7
Applications	IF, IHC, WB
Recommend Dilution	WB 1:2000 IHC 1:50 IF 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human BUB1B expression vector
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	119.5 kDa
Gene Name	BUB1 mitotic checkpoint serine/threonine kinase B
Database Link	NP_001202 Entrez Gene 701 Human UniProt ID O60566
Background	This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Synonyms	Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Cell cycle

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