Rad9 (RAD9A) Mouse Monoclonal Antibody [Clone ID: OTI5D9]

Specifications

Product Data
Clone Name	OTI5D9
Applications	IHC, WB
Recommend Dilution	WB 1:2000 IHC 1:50
Reactivity	Human, Dog, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human RAD9A expression vector
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	42.5 kDa
Gene Name	RAD9 checkpoint clamp component A
Database Link	NP_004575 Entrez Gene 483696 DogEntrez Gene 712345 MonkeyEntrez Gene 5883 Human UniProt ID Q99638
Background	This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.
Synonyms	RAD9
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency

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