PLK1 Mouse Monoclonal Antibody [Clone ID: OTI3F12]

Specifications

Product Data
Clone Name	OTI3F12
Applications	FC, IP, WB
Recommend Dilution	WB 1:2000, IHC 1:150, FLOW 1:100, IP 2ug/500ul
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human PLK1 expression vector
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	68.1 kDa
Gene Name	polo like kinase 1
Database Link	NP_005021 Entrez Gene 18817 MouseEntrez Gene 25515 RatEntrez Gene 5347 Human UniProt ID P53350
Background	Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis
Synonyms	PLK; STPK13
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation

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