GFAP Mouse Monoclonal Antibody [Clone ID: OTI4D11]

Specifications

Product Data
Clone Name	OTI4D11
Applications	IF, WB
Recommend Dilution	WB 1:2000, IHC: 1:50-1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human GFAP expression vector
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	49.9 kDa
Gene Name	glial fibrillary acidic protein
Database Link	NP_002046 Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human UniProt ID P14136
Background	This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Synonyms	ALXDRD
Reference Data
Protein Families	ES Cell Differentiation/IPS

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