Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: OTI3F8]

Specifications

Product Data
Clone Name	OTI3F8
Applications	IF, IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:50 - 1:150, IF 1:100
Reactivity	Human, Dog, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	43.9 kDa
Gene Name	keratin 19
Database Link	NP_002267 Entrez Gene 360626 RatEntrez Gene 100685481 DogEntrez Gene 3880 Human UniProt ID P08727
Background	Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
Synonyms	CK19; K1CS; K19
Reference Data

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