ID2 Mouse Monoclonal Antibody [Clone ID: OTI10C3]

Specifications

Product Data
Clone Name	OTI10C3
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500, IHC 1:50, IF 1:50
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ID2 (NP_002157) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	14.7 kDa
Gene Name	inhibitor of DNA binding 2
Database Link	NP_002157 Entrez Gene 15902 MouseEntrez Gene 25587 RatEntrez Gene 3398 Human UniProt ID Q02363
Background	The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.
Synonyms	bHLHb26; GIG8; ID2A; ID2H
Reference Data
Protein Families	ES Cell Differentiation/IPS, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
Protein Pathways	TGF-beta signaling pathway

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