MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1]

Specifications

Product Data
Clone Name	OTI5E1
Applications	IF, WB
Recommend Dilution	WB: 1:1000, IF (1:100)
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	82.5 kDa
Gene Name	mitogen-activated protein kinase 6
Database Link	NP_002739 Entrez Gene 50772 MouseEntrez Gene 58840 RatEntrez Gene 5597 Human UniProt ID Q16659
Background	ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters
Synonyms	ERK3; HsT17250; p97MAPK; PRKM6
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase

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