Periostin (POSTN) Mouse Monoclonal Antibody [Clone ID: OTI1A1]

Specifications

Product Data
Clone Name	OTI1A1
Applications	IF, WB
Recommend Dilution	WB 1:500, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-230 of human periostin (NP_006466) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	93.2 kDa
Gene Name	periostin
Database Link	NP_006466 Entrez Gene 50706 MouseEntrez Gene 10631 Human UniProt ID Q15063
Background	Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta 1(TGF beta 1) and bone morphogenetic protein (BMP2). Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart. Abnormal expression of periostin is also linked to angiogenesis and metastatsis in epithelial tumors.
Synonyms	OSF-2; OSF2; PDLPOSTN; PN
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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