BrdU Mouse Monoclonal Antibody [Clone ID: OTI2B5]

CAT#: CF190219

Carrier-free (BSA/glycerol-free) BrdU mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)

Formulation: Standard Carrier-Free



  View other "OTI2B5" antibodies (2)

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CNY 4,400.00


货期*
2周

规格
    • 100 ug

经常一起买 (2)
Biotinylation Labelling Kit formatted 4 x 0.05mg
    • 200 ug

CNY 3,170.00


HRP Conjugation kit for 0.2mg IgG
    • 200 ug

CNY 3,230.00

Specifications

Product Data
Clone Name OTI2B5
Applications FC, IF, IHC
Recommend Dilution IHC 1:150, IF 1:150
Host Mouse
Clonality Monoclonal
Immunogen Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Note 5-chloro-2'-deoxyuridine (ClDU), 5-bromo-2'-deoxyuridine (BrdU), 5-iodo-2'-deoxyuridine (IdU) and 5-ethynyl-2'-deoxyuridine (EdU) are nucleoside analogs of thymidine. Cells that treated with these analogs will incorporate the chemicals into the genomic DNA during S-phase. Immunochemical method detection of these analogs is thus used to quantify the cell proliferation, cell cycle status in vitro or in vivo. Since the thymidine analogs can be passed onto the daughter cells, they can also used to trace dividing cell fate in a short period of time over 3 generations. In addition, combination of different analogs and their specific antibodies can be used to trace cell fate in different time frames.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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