BrdU Mouse Monoclonal Antibody [Clone ID: OTI2B5]
CAT#: CF190219
Carrier-free (BSA/glycerol-free) BrdU mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)
Formulation: Standard
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CNY 4,400.00
Specifications
Product Data | |
Clone Name | OTI2B5 |
Applications | FC, IF, IHC |
Recommend Dilution | IHC 1:150, IF 1:150 |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH). |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage Condition | Store at -20°C as received. |
Note | 5-chloro-2'-deoxyuridine (ClDU), 5-bromo-2'-deoxyuridine (BrdU), 5-iodo-2'-deoxyuridine (IdU) and 5-ethynyl-2'-deoxyuridine (EdU) are nucleoside analogs of thymidine. Cells that treated with these analogs will incorporate the chemicals into the genomic DNA during S-phase. Immunochemical method detection of these analogs is thus used to quantify the cell proliferation, cell cycle status in vitro or in vivo. Since the thymidine analogs can be passed onto the daughter cells, they can also used to trace dividing cell fate in a short period of time over 3 generations. In addition, combination of different analogs and their specific antibodies can be used to trace cell fate in different time frames. |
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