Fn1 Rabbit Polyclonal Antibody
CAT#: BP8026
Fn1 rabbit polyclonal antibody, Purified
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CNY 14,063.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, IHC, R |
Recommend Dilution | This antibody can be used on Frozen and Paraffin Embedded Tissues: IFA (1/80), IHC(P) (1/500) After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation time: IHC(P) 60 min at RT or 2-8°C over night. Also suitable for ELISA (1/200) and RIA. |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Purified fibronectin from Murine plasma. |
Specificity | Murine Fibronectin 100% Murine Collagen type I, III and IV all < 0.1% Murine Laminin < 0.1% (RIA at 1/10000 dilution). |
Formulation | PBS without preservative or stabilizer. State: Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore with 0.5 ml distilled water, dilute further with appropriate antibody diluent. |
Concentration | ~1.0 mg/ml (after reconstitution) |
Purification | Affinity Chromatography. |
Conjugation | Unconjugated |
Storage Condition | Sore the antibody undiluted 2-8°C for one month or (in aliquots) at -20°C longer. Avoid repeated freezing and thawing. |
Gene Name | fibronectin 1 |
Database Link | |
Background | Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. |
Synonyms | FN1, Cold-insoluble globulin, CIG |
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