Cytokeratin 8 (KRT8) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting: 1/3000 (ECL method).  Immunohistochemistry on Frozen Sections: 1/50 - 1/250. Immunohistochemistry on Paraffin Sections: 1/50 - 1/250 (after microwave treatment or trypsin).  Immunohistochemistry on Cytological Material.  Incubation Time: 1 h at RT, extended with paraffin sections.  Positive Control: Human intestinal epithelia.
Reactivity	Human, Mouse, Bovine, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide (C-MSIRVTQKSYKVST) of Human keratin K8 (formerly also designated cytokeratin 8), coupled to KLH.
Specificity	Specific for Keratin K8. Marker for discrimination of simple / non-stratified epithelia from those of stratified origin. Basic Human keratin K8 (Mr 52 000) of all simple type and complex epithelia.
Formulation	State: Serum State: Liquid Stabilized Serum Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data

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