Perilipin-1 (PLIN1) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunoblotting (Western blotting): 1/2000 (ECL). Immunohistochemistry on Frozen Tissue: For staining protocols See ref. Ohsaki et al. Immunohistochemistry on Paraffin Sections: 1/100-200 when using microwave treatment. For protocol cf. Straub et al. 2008. Incubation Time: 1h at RT for Immunohistochemistry.
Reactivity	Bovine, Human, Mouse, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Duplicated N-terminus of Perilipin, aa 1-20 (cf. Greenberg et al. 1992, JBC 266, 11341-11346)
Specificity	The antiserum reacts specifically with Perilipins (A and B) located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. It also is a useful pathological marker since PLIN1 becames expressed de novo in hepatocyte steatogenesis.  This antiserum does not cross-react with ADRP (Adipophilin) or TIP47 proteins (additional members of the PAT-family).
Formulation	State: Serum State: Liquid stabilized antiserum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	perilipin 1
Database Link	Entrez Gene 25629 RatEntrez Gene 103968 MouseEntrez Gene 5346 Human UniProt ID O60240
Background	Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, Perilipin A and B, arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kD, respectively. The N-terminus, however, remains unchanged.
Synonyms	Perilipin, PLIN, PLIN1, PLIN-1, PERI
Reference Data
Protein Families	Druggable Genome
Protein Pathways	PPAR signaling pathway

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