Digoxigenin Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (1/100-1/5,000). Western blot. Immunohistochemistry on Frozen Tissue.
Host	Sheep
Clonality	Polyclonal
Immunogen	Digoxin-3-CMO conjugated to KLH
Specificity	The antibody recognizes Digoxigenin. It cross reacts with Digoxin and Digitoxin. Negligible reactivity has been observed with Ouabain.
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G .
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Digoxigenin is a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production. Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
Reference Data

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