VC1456 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP
Recommend Dilution	ELISA: Titer: >1:1,000. Also suitable for conjugation purposes. Immunoprecipitates toxin in gel techniques.
Reactivity	Vibrio cholerae
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified choleragenoid.
Specificity	Beta subunit of Cholera toxin.
Formulation	0.01M PBS, pH 7.2 with 0.09% sodium azide as preservative. State: Purified State: Liquid purified Ig fraction (>95% pure) .
Concentration	lot specific
Purification	Protein A chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P01556
Background	The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
Synonyms	Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, ctxB, toxB, VC_1456
Reference Data

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