EPO Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	ELISA: 0.5-1 µg/ml. Western Blot: 1-2 µg/ml (Reducing and Non-Reducing Conditions). Functional Assays: The ND50 was determined to be approximately 1-3 µg/ml, in the presence of 0.3 units/ml of rhEpo, using a Human factor dependant cell line. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Immunohistochemistry on Paraffin Sections: 15 µg/ml.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	CHO cell-derived, recombinant Human Erythropoietin.
Specificity	This antibody reacts with Erythropoietin, the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified IgG fraction Stabilizer: 5% Trehalose Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	erythropoietin
Database Link	Entrez Gene 2056 Human UniProt ID P01588
Background	Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Synonyms	Epoetin
Reference Data

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