Nes Mouse Monoclonal Antibody [Clone ID: 4D4 (Rat-401)]

Specifications

Product Data
Clone Name	4D4 (Rat-401)
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: BM636 reacts with a band at 200-220 kDa in reducing gels of newborn Rat or Mouse cell extracts. It is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% Glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA; 5% PAGE gels. Immunohistochemistry on Frozen and Paraffin Sections: 1/20-1/200. We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. Immunocytochemistry: We recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM Sodium Borate at pH 9.5.
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Nestin purified from embryonic Rat spinal cord. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
Specificity	This antibody recognizes Mouse and Rat Nestin and does not recognizes Human Nestin.
Formulation	TRIS buffered saline State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nestin
Database Link	Entrez Gene 18008 Mouse UniProt ID Q6P5H2
Background	Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.  This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
Synonyms	NES, Nbla00170
Reference Data

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