Cardiotin Mouse Monoclonal Antibody [Clone ID: SR-2]

Specifications

Product Data
Clone Name	SR-2
Applications	IHC, WB
Recommend Dilution	Immunoblotting.  Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue. Recommended Dilutions: 1/25–1/100 for immunohistochemistry with ABC as detection reagent, and 1/50–1/500 for immunoblotting.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	100 kDa Cardiotin subunit
Specificity	This antibody SR-2 reacts with Cardiotin, a mitochondrionassociated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro.   In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Reference Data

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