Reticulon 1 (RTN1) (Isoform RTN1-A/B) Mouse Monoclonal Antibody [Clone ID: RNL-3]

Specifications

Product Data
Clone Name	RNL-3
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunoblotting: 1/100-1/500. Immunocytochemistry. Flow Cytometry: 1/100-1/200. Immunohistochemistry on Frozen Sections: 1/100-1/200 with ABC as detection reagent. Immunohistochemistry on Paraffin Sections: 1/100-1/200 with ABC as detection reagent.
Reactivity	Human, Monkey, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Small cell lung cancer cell line NCI-H82.
Specificity	RNL-3 recognizes an epitope located within the region of amino acids 421-589 of the neuroendocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-3 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
Formulation	Phosphate buffered saline (PBS) State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	reticulon 1
Database Link	Entrez Gene 6252 Human UniProt ID Q16799
Background	Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon- 1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
Synonyms	Reticulon-1, RTN1, NSP, Neuroendocrine-specific protein
Reference Data

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