CD62E (SELE) Rat Monoclonal Antibody [Clone ID: UZ4]

Specifications

Product Data
Clone Name	UZ4
Applications	FC, FN, IF, IP, WB
Recommend Dilution	Immunoprecipitation. UZ4 is a Functional antibody in that it inhibits leukocyte adhesion to both Murine and Human activated endothelial cells. For in vivo application in mice 20 µg of purified Ig per injection is recommended. UZ4 is also suitable for Immunoblotting, ELISA, Immunoprecipitation and Flow Cytometry (1/500). It is not optimal for Immunohistochemistry; to analyze Mouse E-Selectin protein in Fozen Sctions, please refer to the anti E-Selectin monoclonal antibodies UZ5 (Cat.-No BM6033P), UZ6 (Cat.-No BM6034P), and UZ7 (Cat.-No BM6035P).
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	LPS-activated mlEND1 cells expressing E-Selectin at their cell surface
Specificity	UZ4 recognizes the minimal amino acid consensus sequence CXKKKL present in the lectin domain of both Murine and Human E-Selectin.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	selectin E
Database Link	Entrez Gene 6401 Human UniProt ID P16581
Background	Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF-alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Synonyms	SELE, ELAM1, LECAM2
Reference Data

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